![]() Materials and Methods: We prepared 40 units of RDP by the Buffy Coat method from whole blood stored at room temperature within 8 h of collection (fresh BC) and another 40 units from Buffy Coat stored at 22☌ for less than 24 h before preparation of RDP(stored BC). Advantages of the Buffy Coat method are less activation of the platelets, recovery of more number of platelets and recovery of more volume of plasma for clinical use.Īim: Our aim was to evaluate the platelet count, WBC contamination, platelet activation and other biochemical parameters of platelet concentrate prepared from the Buffy Coat within 8 h and within 24 h of collection, respectively, in order to assess and compare the two methods. In India, when RDP is prepared by the Buffy Coat method, it is done so, almost entirely, from the whole blood stored at room temperature for less than 8 h as per the DGHS India specifications. RDP is prepared entirely by the Buffy Coat method in Europe. Introduction: Random donor platelets (RDPs) are prepared almost entirely by the platelet-rich plasma method in North America. Samantha Kumarage, Joseph Philip, T Chatterjee, Surg Cmdr, R S Mallhi
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